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US 12,263,209 B2Granted
Pharmaceutical compositions comprising collagenase I and collagenase II
AssigneeENDO Biologics, Inc.
InventorWayne K. Herber
Life of this patent
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Abstract
The invention relates to recombinant nucleic acid and polypeptides encoding collagenase I and collagenase II, methods for the preparation thereof and methods for the use thereof. The invention also encompasses methods related to releasing a composition comprising collagenase prior to therapeutic administration.
Claims20
A pharmaceutical composition comprising,
- collagenase I purified from a host cell comprising a vector, the vector comprising a recombinant nucleic acid molecule comprising a polynucleotide encoding the amino acid sequence of SEQ ID NO:
- 5 and a heterologous regulatory sequence operably linked to the polynucleotide;
- and
- collagenase II purified from a host cell comprising a vector, the vector comprising a recombinant nucleic acid molecule comprising a polynucleotide encoding the amino acid sequence of SEQ ID NO:
- 6 and a heterologous regulatory sequence operably linked to the polynucleotide;
- wherein the collagenase I and collagenase II are present in a ratio of about 1 to 1 and the composition is free of hemolytic toxins as measured by a hemolysis assay.
3 more elements
A pharmaceutical composition comprising,
- collagenase I purified from a host cell comprising a vector, the vector comprising a recombinant nucleic acid molecule comprising a polynucleotide encoding the amino acid sequence of SEQ ID NO:
- 5 and a heterologous regulatory sequence operably linked to the polynucleotide;
- and
- collagenase II purified from a host cell comprising a vector, the vector comprising a recombinant nucleic acid molecule comprising a polynucleotide encoding the amino acid sequence of SEQ ID NO:
- 6 and a heterologous regulatory sequence operably linked to the polynucleotide;
- wherein the collagenase I and collagenase II are present in a ratio of about 1 to 1 and the composition is free of a toxin having the amino acid sequence of SEQ ID NO:
- 8, SEQ ID NO:
- 10, SEQ ID NO:
- 12, SEQ ID NO:
- 14, SEQ ID NO:
- 16, or a combination thereof.
8 more elements
Dependencies are inferred from the claim text.