High speed scanning system with acceleration tracking
AssigneePacific Biosciences of California, Inc.
InventorsWindsor Owens, Bryan P. Staker, Robert Hartlage, Edvinas Zizminskas, David Stern, Paul Heilman
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Abstract
Disclosed herein is a high throughput optical scanning device and methods of use. The optical scanning device and methods of use provided herein can allow high throughput scanning of a continuously moving object with a high resolution despite fluctuations in stage velocity. This can aid in high throughput scanning of a substrate, such as a biological chip comprising fluorophores. Also provided herein are improved optical relay systems and scanning optics.
Claims20
A system comprising:
- (a) a stage, wherein, when in use, said stage (i) moves along an axis and (ii) holds a substrate comprising a plurality of fields;
- (b) an objective lens;
- (c) a camera optically coupled to said stage by a light path, wherein, when in use, said camera captures an image of one field of said plurality of fields through said objective lens, said image acquired via at least a portion of said light path, wherein said portion is defined from one field of said plurality of fields through said objective lens to said camera during acquisition of said image;
- (d) a motion tracking mirror mounted along said light path;
- (e) an electric motor operably coupled to said motion tracking mirror to actuate angular motion of said motion tracking mirror along said axis of stage movement in said light path;
- and
- (f) a controller module operably coupled to said electric motor to send a driving signal to said electric motor, wherein, when in use, said controller module generates said driving signal as a function of a velocity fluctuation of said stage or said substrate movement along said axis.
A method of imaging a plurality of fields on a moving substrate, comprising:
- (a) providing an optical scanning system comprising:
- (i) a stage, wherein said stage holds a substrate comprising a plurality of fields;
- (ii) an objective lens;
- (iii) a camera optically coupled to said stage by a light path;
- (iv) a motion tracking mirror mounted along said light path;
- (v) an electric motor operably coupled to said motion tracking mirror to actuate angular motion of said motion tracking mirror along an axis of stage movement in said light path;
- and
- (vi) a controller module operably coupled to said electric motor to send a driving signal to said electric motor, wherein said controller module generates said driving signal as a function of a velocity fluctuation of said stage or said substrate movement along said axis;
- (b) moving said substrate with said stage along said axis;
- (c) actuating said electric motor operably coupled to said motion tracking mirror with said driving signal;
- and
- (d) capturing an image of a field of said plurality of fields with said camera through said objective lens, wherein said image is captured via at least a portion of said light path, and wherein said at least said portion of said light path is defined from one of said plurality of fields through said objective lens to said camera while said image is being captured.
Dependencies are inferred from the claim text.