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US 12,259,396 B2Granted
Methods and compositions for assessing antibody specificities
AssigneeSerimmune Inc.
InventorsPatrick Sean Daugherty, Kathryn Vinaya Louise Kamath, Jack Ryan Reifert
Life of this patent
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Abstract
The present invention provides compositions and methods that can be used to determine a peptide signature for an antibody repertoire in a sample comprising multiple antibodies. The method can be used to characterize a phenotype in a sample, such as providing a diagnosis, prognosis or theranosis of a medical condition.
Claims30
An array comprising a peptide comprising a binding motif sequence [LI]xxA[ILV]xxRG (SEQ ID NO:
- 362), [DA]DPTN (SEQ ID NO:
- 367), or KETPPALN (SEQ ID NO:
- 421), wherein a length of the peptide comprising the binding motif sequence is 30 amino acids or less, and wherein the peptide is operably linked to an array surface to present the peptide for binding by an antibody, wherein the array surface comprises:
- (i) a solid surface, wherein the peptide is attached to the solid surface, or
- (ii) a biological particle, wherein the biological particle is engineered to express the peptide operably linked to a display scaffold, wherein the display scaffold is configured to display the peptide on the outer surface of the biological particle, and wherein the amino acid sequence of the peptide is heterologous to the biological particle.
2 more elements
An array comprising:
- (i) a peptide comprising a binding motif sequence [LI]xxA[ILV]xxRG (SEQ ID NO:
- 362), [DA]DPTN (SEQ ID NO:
- 367), or KETPPALN (SEQ ID NO:
- 421), wherein a length of the peptide comprising the binding motif sequence [LI]xxA[ILV]xxRG (SEQ ID NO:
- 362), [DA]DPTN (SEQ ID NO:
- 367), or KETPPALN (SEQ ID NO:
- 421) is 30 amino acids or less;
- and
- (ii) a peptide comprising a second binding motif selected from the group consisting of:
- LXGM[RQ]K (SEQ ID NO:
- 365), QEG[IV]Q (SEQ ID NO:
- 360), Q[TI]EQxxxxxK (SEQ ID NO:
- 371), VxxYFxx[LV]xK (SEQ ID NO:
- 374), PFx[AP]YxK (SEQ ID NO:
- 373), DxSP[IL]E (SEQ ID NO:
- 372), KxVDxDR (SEQ ID NO:
- 375), and [DN][AS]A[AG]F (SEQ ID NO:
- 376),
- wherein the peptide comprising the binding motif sequence [LI]xxA[ILV]xxRG (SEQ ID NO:
- 362), [DA]DPTN (SEQ ID NO:
- 367), or KETPPALN (SEQ ID NO:
- 421) and the peptide comprising the second binding motif sequence are each operably linked to an array surface to present the peptides for binding by one or more antibodies, wherein the array surface comprises:
- (i) a solid surface, wherein the peptide comprising the binding motif sequence [LI]xxA[ILV]xxRG (SEQ ID NO:
- 362), [DA]DPTN (SEQ ID NO:
- 367), or KETPPALN (SEQ ID NO:
- 421) and the peptide comprising the second binding motif sequence are each attached to the same solid surface, or
- (ii) a first biological particle, wherein the first biological particle is engineered to express the peptide comprising the binding motif sequence [LI]xxA[ILV]xxRG (SEQ ID NO:
- 362), [DA]DPTN (SEQ ID NO:
- 367), or KETPPALN (SEQ ID NO:
- 421), and a second biological particle, wherein the second biological particle is engineered to express the peptide comprising the second binding motif sequence, wherein each biological particle expresses each respective peptide operably linked to a display scaffold, wherein the display scaffold is configured to display the respective peptides on the outer surface of the respective biological particle, and wherein the amino acid sequence of the peptide is heterologous to the respective biological particle.
27 more elements
Dependencies are inferred from the claim text.