Methods and compositions for protein sequencing
AssigneeQuantum-Si Incorporated
InventorsBrian Reed, Jeremy Lackey, Haidong Huang
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Abstract
Aspects of the application provide methods of identifying and sequencing proteins, polypeptides, and amino acids, and compositions useful for the same. In some aspects, the application provides methods of obtaining data during a degradation process of a polypeptide, and outputting a sequence representative of the polypeptide. In some aspects, the application provides amino acid recognition molecules having a shielding element that enhances photostability in polypeptide sequencing reactions.
Claims29
A method of polypeptide sequencing, the method comprising:
- contacting a single polypeptide molecule with one or more terminal amino acid recognition molecules, wherein at least one of the one or more terminal amino acid recognition molecules that is contacted with the single polypeptide molecule is an SH2 domain-containing protein;
- and
- detecting a series of signal pulses indicative of association of the one or more terminal amino acid recognition molecules with successive amino acids exposed at a terminus of the single polypeptide while the single polypeptide is being degraded, thereby sequencing the single polypeptide molecule,
- wherein sequencing comprises identifying that an amino acid of the single polypeptide molecule comprises a phosphorylated side chain based at least in part on a characteristic pattern in the series of signal pulses.
A method of polypeptide sequencing, the method comprising:
- contacting a single polypeptide molecule with one or more terminal amino acid recognition molecules, wherein at least one of the one or more terminal amino acid recognition molecules that is contacted with the single polypeptide molecule is a recognition protein comprising an amino acid sequence that is at least 90% identical to any one of SEQ ID NOs:
- 22-25;
- and
- detecting a series of signal pulses indicative of association of the one or more terminal amino acid recognition molecules with successive amino acids exposed at a terminus of the single polypeptide while the single polypeptide is being degraded, thereby sequencing the single polypeptide molecule,
- wherein sequencing comprises identifying that an amino acid of the single polypeptide molecule comprises a phosphorylated side chain based at least in part on a characteristic pattern in the series of signal pulses.
Dependencies are inferred from the claim text.