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US 12,259,383 B2Granted
Cho host cell protein assay
AssigneeCharles River Laboratories International, Inc.
InventorOlaf Stamm
Life of this patent
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Abstract
The present invention provides compositions, kits, and methods for the detection of host cell proteins (HCPs) in biological samples. In some embodiments, the present invention utilizes immunization of aves hosts with proteins derived from non-aves host cells to produce aves antibodies specific for non-aves HCPs.
Claims12
A kit comprising one or more host cell protein (HCP)-specific antibodies for use in the detection of one or more HCPs in a biological sample,
- wherein the HCPs are derived from a non-aves host,
- wherein the one or more HCP-specific antibodies are produced in an aves host and are an IgY isotype,
- wherein the one or more HCP-specific antibodies are capable of detecting at least 85% of the HCP proteins present in the sample,
- wherein the one or more HCP-specific antibodies comprise a detectable label, and
- wherein the detectable label is biotin, an enzyme label or a fluorescent label.
2 more elements
A kit comprising a first and a second host cell protein (HCP)-specific antibody for use in the detection of one or more HCPs in a biological sample,
- wherein the HCPs are derived from a non-aves host,
- wherein the first and the second HCP-specific antibodies are produced in an aves host and are an IgY isotype, and
- wherein the first HCP-specific antibody is capable of detecting at least 85% of the HCP proteins present in the sample,
- wherein the second HCP-specific antibody comprises a detectable label, and
- wherein the detectable label is biotin, an enzyme label, or a fluorescent label.
2 more elements
Dependencies are inferred from the claim text.