Antigen detection method and kit with false positive signal removed
AssigneeJl Medilabs, Inc
InventorJong Jin Lee
Life of this patent
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Abstract
A kit for detecting an antigen from which false positive signals are removed is provided. The kit includes a substrate; a capture antibody attachable onto the substrate and having a capture strand labeled with a fluorescent material; a detection antibody having a detection strand labeled with a fluorescent material and complementary to some or all of a base sequence of the capture strand; and a blocking strand having a base sequence capable of complementary binding to the capture strand or the detection strand to prevent the detection strand and the capture strand from complementary binding to each other.
Claims11
A kit for detecting an antigen from which false positive signals are removed, comprising:
- a substrate;
- a capture antibody attachable onto the substrate and having a capture strand labeled with a fluorescent material;
- a detection antibody having a detection strand labeled with a fluorescent material and complementary to some or all of a base sequence of the capture strand;
- and
- a blocking strand having a base sequence capable of complementary binding to the capture strand or the detection strand to prevent the detection strand and the capture strand from complementary binding to each other.
A method for detecting an antigen from which false positive signals are removed, comprising:
- (a) preparing a capture antibody having a capture strand labeled with a fluorescent material;
- (b) preparing a detection antibody having a detection strand labeled with a fluorescent material and complementary to some or all of a base sequence of the capture strand;
- (c) allowing a blocking strand to complementarily bind to one or more of the capture strand and the detection strand;
- (d) attaching the capture antibody onto a substrate;
- (e) introducing a sample comprising an antigen to induce an antigen-antibody reaction among the antigen, the capture antibody, and the detection antibody;
- (f) removing the blocking strand to allow the detection strand to complementarily bind to the capture strand;
- and
- (g) measuring fluorescence signals generated through the binding of the detection strand to the capture strand.
Dependencies are inferred from the claim text.