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US 12,258,630 B2Granted
Methods and systems for assessing and/or quantifying sperm cell subpopulations bearing a specific genetic signature
AssigneeABS Global, Inc.
InventorsElon Roti-Roti, Nicole Cray, Matthias Wagner, Michael Reid Botts
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Abstract
Technologies for assessing, quantifying and isolating sperm cell populations and/or subpopulations having specific genetic signatures are provided, as well as methods and systems to assess the efficacy of chromosomal differentiation processes. Compositions for identification and differentiation of X-chromosomes and Y-chromosomes in DNA are also provided.
Claims20
A system for assessing the sex-skew in a population of cells, comprising:
- a first oligonucleotide agent that selectively binds to an X-chromosome nucleic acid sequence comprising at least one sequence selected from the group consisting of SEQ ID NO:
- 4-6, 28-35, 222, and 845-847;
- and
- a second oligonucleotide agent that selectively binds to a Y-chromosome nucleic acid sequence comprising at least one sequence selected from the group consisting of SEQ ID:
- 7-9, 36-55, 767, 850-861, and 864-875.
2 more elements
A method of assessing sex-skew in a population of sperm cells, the method comprising steps of:
- detecting binding of a set of oligonucleotide agents to sperm DNA obtained from a sample of sperm cells, wherein the set includes:
- a first oligonucleotide agent that selectively binds to an X-chromosome nucleic acid sequence comprising at least one sequence selected from the group consisting of SEQ ID NO:
- 4-6, 28-35, 222, and 845-847;
- and
- a second oligonucleotide agent that selectively binds to a Y-chromosome nucleic acid sequence comprising at least one sequence selected from the group consisting of SEQ ID:
- 7-9, 36-55, 767, 850-861, and 864-875.
3 more elements
Dependencies are inferred from the claim text.