Methods for a controlled coenzyme Q10 fermentation production process

A controlled fermentation production process, optimized for producing coenzyme Q10 (CoQ10), comprising:

• growing a microbial culture for a predetermined period of time in a medium in a tank, thereby producing CoQ10-containing bacteria, wherein for optimized results, the process further comprises:
• (1) during 0-16 h time period of growing the microbial culture, maintaining a tank pressure between 0.01-0.1 MPa for more than 80% of the 0-16 h time period to keep at least one of a concentration of carbon dioxide in an exhaust gas of the microbial culture at 0.5%-13% or a concentration of lactate in the microbial culture at 5-75 mg/L;
• (2) during 16-48 h time period of growing the microbial culture, maintaining the tank pressure between 0.01-0.1 MPa for more than 80% of the 16-48 h time period to keep at least one of the concentration of carbon dioxide at 3%-15% or the concentration of lactate at 10-120 mg/L;
• (3) during 48-80 h time period of growing the microbial culture, maintaining the tank pressure between 0.01-0.1 MPa for more than 80% of the 48-80 h time period to keep at least one of the concentration of carbon dioxide at 3%-12% or the concentration of lactate at 20-180 mg/L;
• and
• (4) during a time period after 80 h of growing the microbial culture, maintaining the tank pressure between 0.01-0.1 MPa for more than 80% of the time period after 80 h to keep at least one of the concentration of carbon dioxide at 2%-10% or the concentration of lactate at 40-120 mg/L.

A controlled fermentation production process, optimized for producing coenzyme Q10 (CoQ10), comprising:

• growing a microbial culture for a predetermined period of time in a medium in a tank and providing an air supply that includes a carbon source, thereby producing CoQ10-containing bacteria, wherein for optimized results, the process further comprises:
• (1) during 0-16 h time period of growing the microbial culture, maintaining an air supply rate between 0.1-1.1 VVM for more than 80% of the 0-16 h time period to keep at least one of a concentration of carbon dioxide in an exhaust gas of the microbial culture at 0.5%-13% or a concentration of lactate in the microbial culture at 5-75 mg/L;
• (2) during 16-48 h time period of growing the microbial culture, maintaining the air supply rate between 0.1-1.1 VVM for more than 80% of the 16-48 h time period to keep at least one of the concentration of carbon dioxide at 3%-15% or the concentration of lactate at 10-120 mg/L;
• (3) during 48-80 h time period of growing the microbial culture, maintaining the air supply rate between 0.1-1.1 VVM for more than 80% of the 48-80 h time period to keep at least one of the concentration of carbon dioxide at 3%-12% or the concentration of lactate at 20-180 mg/L;
• and
• (4) during a time period after 80 h of growing the microbial culture, maintaining the air supply rate between 0.1-1.1 VVM for more than 80% of the time period after 80 h to keep at least one of the concentration of carbon dioxide at 2%-10% or the concentration of lactate at 40-120 mg/L.