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US 12,258,601 B2Granted
Enzymatic production of tagatose
AssigneeBonumose, Inc.
InventorDaniel Joseph Wichelecki
Life of this patent
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Abstract
Disclosed herein are improved processes for making tagatose including the steps of converting F6P to T6P, catalyzed by a F6PE; and converting the T6P to tagatose, catalyzed by a T6PP, using enzymes with higher activities compared to F6PEs and T6PPs previously used in a process to produce tagatose.
Claims18
An improved process for the production of tagatose from a saccharide, the improved process comprising:
- converting fructose-6-phosphate (F6P) to tagatose 6-phosphate (T6P) using a fructose 6-phosphate epimerase (F6PE) wherein the F6PE is selected from an amino acid sequence having at least 90% sequence identity to SEQ ID NO:
- 2 or SEQ ID NO:
- 7;
- and
- converting T6P to tagatose using a tagatose 6-phosphate phosphatase (T6PP),
- wherein the F6PE has a higher activity in the improved process compared to that of F6PE from Dictyoglomus thermophilum (Uniprot ID B5YBD7).
3 more elements
An improved process for the production of tagatose from a saccharide, the improved process comprising:
- converting fructose-6-phosphate (F6P) to tagatose 6-phosphate (T6P) using a fructose 6-phosphate epimerase (F6PE);
- and
- converting T6P to tagatose using a tagatose 6-phosphate phosphatase (T6PP), wherein the T6PP is selected from an amino acid sequence having at least 90% sequence identity to SEQ ID NO:
- 3, SEQ ID NO:
- 4, SEQ ID NO:
- 5, or SEQ ID NO:
- 6,
- wherein the T6PP has a higher activity in the improved process compared to that of T6PP from Archaeoglobus fulgidus (Uniprot ID 029805).
5 more elements
Dependencies are inferred from the claim text.