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US 12,258,590 B2Granted
Mutant Taq polymerase providing faster amplification
AssigneeAbClonal Science, Inc.
InventorsZhenyu Zhu, Dapeng Sun
Life of this patent
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Abstract
The invention includes a mutant Taq polymerase, which can significantly extend and amplify a target sequence where the extension conditions are time limited to as little as one second. The mutant Taq polymerase, or a biologically active fragment thereof, has one or more substitutions differing from the wild type as shown in Table I.
Claims4
A mutant Taq polymerase comprising:
- the following amino acid mutations shown in the following even-numbered sequences, and wherein the remainder of the mutant polymerase has at least 80% amino acid sequence identity with wild type Taq polymerase, as shown in SEQ ID NO:
- 4, but not including the 6-membered histidine tag at its C-terminus and the six immediately preceding Glycine and Serine amino acids shown in SEQ ID NO:
- 4:
- E189L (SEQ ID NOS:
- 102-103), E189M (SEQ ID NOS:
- 104-105), E189P (SEQ ID NOS:
- 106-107), E189Q (SEQ ID NOS:
- 108-109), E189R (SEQ ID NOS:
- 110-111), E189T (SEQ ID NOS:
- 112-113), E189W (SEQ ID NOS:
- 114-115), E201K (SEQ ID NOS:
- 116-117), E230C (SEQ ID NOS:
- 122-123), E230F (SEQ ID NOS:
- 124-125), E230H (SEQ ID NOS:
- 126-127), E230K/E520K (SEQ ID NOS:
- 132-133), E230K/E537K (SEQ ID NOS:
- 134-135), E230K/D578R (SEQ ID NOS:
- 136-137), and E230K/D732R (SEQ ID NOS:
- 138-139).
16 more elements
Dependencies are inferred from the claim text.