Fusion polypeptides for production of 7-dehydrocholesterol and methods of use thereof
AssigneeHangzhou Enhe Biotechnology Co., Ltd.
InventorsYazhou Zu, Zhenhua Pang, Igor Walter Bogorad
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Abstract
The present disclosure relates to fusion proteins including an iron-sulfur protein (e.g., cholesterol 7-desaturase (C7D)) and a ferredoxin reductase (e.g., KshB) that can efficiently convert cholesterol to 7-dehydrocholesterol (7-DHC) in vivo. Also disclosed herein are engineered microbes (e.g., E. coli) expressing the fusion proteins and methods of use thereof.
Claims20
A fusion polypeptide, comprising a first moiety comprising an iron-sulfur protein that can convert cholesterol to 7-dehydrocholesterol (7-DHC), and a second moiety comprising a ferredoxin reductase.
A polynucleotide comprising from 5′ end to 3′ end:
- (a) a first nucleic acid encoding an iron-sulfur protein that can convert cholesterol to 7-dehydrocholesterol (7-DHC),
- (b) optionally a second nucleic acid encoding a linker;
- and
- (c) a third nucleic acid encoding a ferredoxin reductase.
Dependencies are inferred from the claim text.