Mammalian cell population and medicaments for cell therapies in humans and improved cell cultivation methods
AssigneeScarcell Technologies
InventorsAntoine Lafont, Mathieu Castela
Life of this patent
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Abstract
Compositions of matter, including cell populations and medicaments, are provided that are derived from human gingival fibroblasts and have cell phenotypes that occur in proportions not found in natural gum tissue, but rather, are preferentially are selected to express proteins favoring angiogenesis and anti-inflammatory effects, while reducing cell populations that promote tumorigenicity and/or formation of metalloproteinases that inhibit tissue regeneration. Methods of generating such compositions are provided that increase proliferation many-fold compared to previously known methods, and methods of using such compositions in a wide range of human cell therapies are provided.
Claims13
A composition comprising a conditioned medium derived from a cultured human gingival fibroblast cell population, sodium hyaluronate, sodium chloride, disodium phosphate and monosodium phosphate, wherein the cultured human gingival fibroblast cell population is cultivated so that at least about 90% of the cells of the population express CD90 and CD63 and about 20% or less of the cells of the population express CD146, and wherein the conditioned medium comprises paracrine secretions by the human gingival fibroblast cell population cultured in a medium comprising a buffering agent, L-glutamine, sodium bicarbonate, sodium pyruvate, hypoxanthine, thymidine, and growth factors, for at least 24 hours.
A composition comprising a conditioned medium derived from a cultured human gingival fibroblast cell population, sodium hyaluronate, sodium chloride, disodium phosphate and monosodium phosphate, wherein the cultured human gingival fibroblast cell population is cultivated so that at least about 50% of the cells of the population express CD90 and CD63 and about 10% or less of the cells of the population express CD146, and wherein the conditioned medium comprises paracrine secretions by the human gingival fibroblast cell population cultured in a medium comprising a buffering agent, L-glutamine, sodium bicarbonate, sodium pyruvate, hypoxanthine, thymidine, and growth factors, for at least 24 hours.
Dependencies are inferred from the claim text.