Systems and methods for a collapsible chamber with foldable mixing element
AssigneeLife Technologies Corporation
InventorStephan Kaiser
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Abstract
The present set of embodiments relate to a system, method, and apparatus for culturing cells within a cell culture vessel having a mixing element. The cell culture system includes a flexible portion and a mixing element disposed therein. The mixing element includes a suspended foldable portion. The system is configured to reduce shear stress on cells without compromising mixing efficiency. This reduction is accomplished by using a mixing element having a large surface area allowing for reduced rotational speeds. The system is collapsible for ease of transport and disposal. The flexible portion collapses and the foldable portion folds to minimize the volume of the system while not in operation.
Claims12
A method of culturing cells using a collapsible cell culture vessel, comprising:
- providing a flexible portion containing nutrients that support in vitro cultivation of cells;
- providing a mixing element disposed within the flexible portion to mix a cell culture, wherein the mixing element comprises a foldable sheet, the foldable sheet comprising a protrusion configured to create vigorous flow of a fluid within the flexible portion;
- inflating the flexible portion;
- and
- unfolding the foldable sheet.
Dependencies are inferred from the claim text.