Cross pollination through liquid-mediated delivery of pollen to enclosed stigmas of flowers from recipient plants
AssigneeMonsanto Technology LLC
InventorsHuachun Wang Larue, Li Lin
Life of this patent
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Abstract
The invention provides novel methods for liquid-mediated delivery of pollen grains to enclosed stigmas in recipient female flowers. For example, methods for liquid-mediated pollination are provided. The methods provided include collecting pollen from a donor plant, suspending the collected pollen in a liquid solution, and introducing said solution to an enclosed stigma of a recipient flower bud on a recipient plant, thereby pollinating the flower with the pollen from the donor plant.
Claims11
A method of producing a pollen suspension injection solution comprising a desired pollen concentration for cross-pollination comprising the steps of:
- a) collecting pollen-shedding flowers from a male parent;
- b) homogenizing said flowers to release pollen;
- c) purifying pollen from said homogenized flowers by removing floral debris;
- d) quantifying said purified pollen;
- e) suspending said purified pollen in an injection solution to produce the pollen suspension injection solution comprising a desired pollen concentration, wherein the pollen suspension injection solution comprises 10% to 20% sucrose by weight;
- 0.04% to 0.08% xanthan gum by weight;
- and 1.5 units/L to about 1500 units/L pectin methylesterase (PME),
- f) filling a syringe with the pollen suspension injection solution, and
- g) injecting the pollen suspension injection solution into unopened flower buds.
Dependencies are inferred from the claim text.