Mass spectrometric quantitation assay for metabolites of leflunomide
AssigneeQuest Diagnostics Investments Incorporated
InventorKarin O. Thomassian
Life of this patent
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Abstract
A method for determining an amount of teriflunomide in a sample by mass spectrometry includes adding an internal standard to the sample, subjecting the sample to solid phase extraction, purifying the sample by high turbulence liquid chromatography, ionizing the sample to produce one or more teriflunomide ions detectable by mass spectrometry, determining the amount of the one or more teriflunomide ions by mass spectrometry, and using the amount of the one or more ions determined to further determine the amount of teriflunomide in the sample.
Claims9
A method for determining an amount of teriflunomide in a sample by mass spectrometry, said method comprising:
- a. adding an internal standard to the sample;
- b. subjecting the sample to solid phase extraction;
- c. purifying the sample by high turbulence liquid chromatography;
- d. ionizing the sample to produce one or more teriflunomide ions detectable by tandem mass spectrometry;
- e. determining the amount of the one or more teriflunomide ions by tandem mass spectrometry, wherein the tandem mass spectrometry comprises fragmenting a precursor ion with a mass to charge ratio (m/z) of 271.3±0.50 into one or more fragment ions comprising ions with m/z of 162.1±0.50;
- and
- f. using the amount of the one or more teriflunomide ions determined in step (e) to determine the amount of teriflunomide in the sample.
Dependencies are inferred from the claim text.