Diagnosis method of multiple sclerosis

A method for diagnosing in vitro a central nervous system disease in an individual suspected to be afflicted by such a disease, said method comprising:

• a) measuring the amount of at least one first protein, one second protein and one third protein,
• said first protein comprising the amino acid sequence as set forth in SEQ ID NO:
• 1,
• said second protein comprising the amino acid sequence as set forth in SEQ ID NO:
• 2,
• said third protein comprising the amino acid sequence as set forth in SEQ ID NO:
• 3,
• said at least one first protein, one second protein and one third protein belonging to the group of proteins consisting of a first protein, a second protein, a third protein, a fourth protein and a fifth protein,
• said first protein comprising the amino acid sequence as set forth in SEQ ID NO:
• 1,
• said second protein comprising the amino acid sequence as set forth in SEQ ID NO:
• 2,
• said third protein comprising the amino acid sequence as set forth in SEQ ID NO:
• 3,
• said fourth protein comprising the amino acid sequence as set forth in SEQ ID NO:
• 4,
• said fifth protein comprising the amino acid sequence as set forth in SEQ ID NO:
• 5,
• b) comparing the amount of each of said at least one first protein, one second protein and one third protein with the amount of the same proteins in a biological sample of the same nature originating from a healthy individual not afflicted by a central nervous system disease, to establish the respective protein expression level ratios for each of said at least one first protein, one second protein and one third protein, and
• c) diagnosing said individual as having:
• (i) multiple sclerosis for the ratios of established in step b):
• (1) for said at least first protein is higher than, or equal to, 2,
• (2) for said at least second protein is higher than, or equal to, 2.5, and
• (3) for said at least third protein is higher than, or equal to, 1.5,
• (ii) multiple sclerosis or an inflammatory neurological disease for the ratio established in step b) for said at least the second protein is higher than, or equal to, 2;
• or
• (iii) a clinically isolated syndrome without conversion to multiple sclerosis, a clinically isolated syndrome with conversion to multiple sclerosis, relapsing-remitting multiple sclerosis, primary progressive multiple sclerosis, a non inflammatory neurological disease, an inflammatory neurological disease or peripheral inflammatory neurological disease for the ration established in step b) for said at least third protein is higher than, or equal to, 1.5.

A kit comprising a set of at least 3 antibodies liable to form a protein complex with at least one first protein, one second protein and one third protein,

• said first protein comprising the amino acid sequence as set forth in SEQ ID NO:
• 1,
• said second protein comprising the amino acid sequence as set forth in SEQ ID NO:
• 2,
• said third protein comprising the amino acid sequence as set forth in SEQ ID NO:
• 3,
• said at least one first protein, one second protein and one third protein belonging to the group of proteins consisting of a first protein, a second protein, a third protein, a fourth protein and a fifth protein,
• said first protein comprising the amino acid sequence as set forth in SEQ ID NO:
• 1,
• said second protein comprising the amino acid sequence as set forth in SEQ ID NO:
• 2,
• said third protein comprising the amino acid sequence as set forth in SEQ ID NO:
• 3,
• said fourth protein comprising the amino acid sequence as set forth in SEQ ID NO:
• 4,
• said fifth protein comprising the amino acid sequence as set forth in SEQ ID NO:
• 5.