Back to results
US 12,253,517 B2Granted
Nano-enzyme containers for test elements
AssigneeRoche Diabetes Care, Inc.
InventorsGrit Baier, Carina Horn, Katharina Landfester, Anna Musyanovych, Umaporn Paiphansiri
Life of this patent
Estimating the term…
Abstract
The present invention relates to a test element for the detection of an analyte comprising an enzyme, wherein the enzyme is incorporated in a nanocapsule.
Claims12
A diagnostic test element for the detection of an analyte comprising:
- an enzyme;
- and
- a silica nanocapsule substantially impermeable for the enzyme but permeable for an enzyme substrate, the enzyme being incorporated within the interior of the silica nanocapsule, the interior of the silica nanocapsule being free from a hydrophilic polymer,
- wherein the silica nanocapsule comprises a polyaddition and/or polycondensation product of monomers formed at the interface in a water-in-oil miniemulsion comprising a continuous oil phase and a discontinuous aqueous phase dispersed therein, the enzyme being present in the aqueous phase, and
- wherein the silica nanocapsule is a polycondensation product of a tetra-alkoxy silane with a compound RSiX3,
- wherein X is halo and
- R is an optionally substituted C3-C30alkyl group.
4 more elements
A diagnostic test element for the detection of an analyte comprising:
- an enzyme;
- and
- a silica nanocapsule being substantially impermeable for the enzyme but permeable for an enzyme substrate, the enzyme being incorporated within the interior of the silica nanocapsule, the interior of the silica nanocapsule being free from a structuring surfactant,
- wherein the silica nanocapsule comprises a polyaddition and/or polycondensation product of monomers formed at the interface in a water-in-oil miniemulsion comprising a continuous oil phase and a discontinuous aqueous phase dispersed therein, the enzyme being present in the aqueous phase, and
- wherein the silica nanocapsule is a polycondensation product of a tetra-alkoxy silane with a compound RSiX3,
- wherein X is halo and
- R is an optionally substituted C3-C30 alkyl group.
4 more elements
Dependencies are inferred from the claim text.