DNA modifying enzymes and active fragments and variants thereof and methods of use
AssigneeLife Edit Therapeutics, Inc.
InventorsTyson D. Bowen, Alexandra Briner Crawley, Tedd D. Elich
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Abstract
Compositions and methods comprising novel deaminase polypeptides for targeted editing of nucleic acids are provided. Compositions comprise deaminase polypeptides. Also provided are fusion proteins comprising a DNA-binding polypeptide and a deaminase of the invention. The fusion proteins include RNA-guided nucleases fused to deaminases, optionally in complex with guide RNAs. Compositions also include nucleic acid molecules encoding the deaminases or the fusion proteins. Vectors and host cells comprising the nucleic acid molecules encoding the deaminases or the fusion proteins are also provided.
Claims20
A fusion protein comprising a Type II CRISPR-Cas protein nickase and a deaminase, wherein said deaminase comprises an amino acid sequence having at least 90% sequence identity to the amino acid sequence of SEQ ID NO:
- 405 and has adenine deaminase activity, and wherein said nickase
- (a) is a Cas9 nickase;
- or
- (b) comprises an amino acid sequence having at least 95% sequence identity to any one of SEQ ID NO:
- 42, 52, 53, 55-59, 61, 397, or 398.
A polypeptide having adenine deaminase activity and comprising an amino acid sequence having at least 90% sequence identity to the amino acid sequence of SEQ ID NO:
- 405.
Dependencies are inferred from the claim text.