Strain and method for producing rosmarinic acid
AssigneeHong-Taoism Research Institute of analytical science and technology LTD., Shaanxi Province
InventorsYujie Cai, Yi Yan, Yanrui Ding, Yajun Bai, Xiaohui Zheng
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Abstract
The disclosure discloses a strain and method for producing rosmarinic acid, and belongs to the technical field of bioengineering. The disclosure constructs a recombinant cell or a combination of recombinant cells expressing 4-coumarate: CoA ligase, rosmarinic acid synthase, polyphosphate kinase 2-I (PPK2-I) and polyphosphate kinase 2-II (PPK2-II), and utilizes the recombinant cell or the combination of recombinant cells to catalyze Danshensu and caffeic acid for synthesizing rosmarinic acid. The disclosure has good industrial application prospects.
Claims13
A recombinant microbial cell that synthesizes rosmarinic acid from phenolic acids as substrates, comprising:
- a recombinant Escherichia coli cell comprising genes that encode and are capable of expressing 4-coumarate:
- coenzyme A (CoA) ligase, rosmarinic acid synthase, polyphosphate kinase 2-I (PPK2-I), and polyphosphate kinase 2-II (PPK2-II);
- wherein the gene encoding the 4-coumarate:
- CoA ligase is selected from the group consisting of:
- sb4cl, ot4cl, ob4cl, pc4cl, sc4cl, and rj4cl;
- wherein the gene encoding the rosmarinic acid synthase is selected from the group consisting of:
- psras, laras, moras, smras, ccras, ntras, and dcras;
- wherein the gene encoding the PPK2-I is smpkk;
- and
- wherein the gene encoding the PPK2-II is ajpkk.
Two or more recombinant cells that synthesize rosmarinic acid from phenolic acids as substrates, comprising:
- two or more recombinant cells that are Escherichia coli cells that comprise genes that encode and are capable of expressing one or more of 4-coumarate:
- CoA ligase, rosmarinic acid synthase, polyphosphate kinase 2-II (PPK2-II), and polyphosphate kinase 2-I (PPK2-I);
- wherein each recombinant E. coli cell does not repeatedly express one of the 4-coumarate:
- CoA ligase, the rosmarinic acid synthase, the PPK2-II, and the PPK2-I expressed by the other recombinant E. coli cells;
- wherein the gene encoding the 4-coumarate:
- CoA ligase is selected from the group consisting of:
- sb4cl, ot4cl, ob4cl, pc4cl, sc4cl, and rj4cl;
- wherein the gene encoding the rosmarinic acid synthase is selected from the group consisting of:
- psras, laras, moras, smras, ccras, ntras, and dcras;
- wherein the gene encoding the PPK2-I is smpkk;
- and
- wherein the gene encoding the PPK2-II is ajpkk.
Dependencies are inferred from the claim text.