Use of engineered liver tissue constructs for modeling liver disorders
AssigneeOrganovo, Inc.
InventorsDeborah Lynn Greene Nguyen, Dwayne E. Carter, Rhiannon Hardwick, Alice Chen Walters, Sharon C. Presnell, Chirag B. Khatiwala, Rami Nasrallah
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Abstract
The invention is directed to three-dimensional, engineered, bioprinted biological tissue constructs exhibiting a liver disorder, methods of making the constructs, and use of the constructs in assays, such as drug testing and molecular diagnostic testing, including methods of assessing the ability of a candidate therapeutic agent to reverse, reduce, or prevent a liver disorder, and methods for biomarker discovery.
Claims15
A culture comprising a three-dimensional, engineered, bioprinted, biological liver tissue construct and a culture medium, wherein the liver tissue construct comprises parenchymal cells and non-parenchymal cells, the parenchymal cells comprising hepatocytes and the non-parenchymal cells comprising endothelial cells and hepatic stellate cells, and wherein the culture medium comprises:
- a palmitic acid, an oleic acid, or a combination thereof in a concentration that is capable of inducing a nonalcoholic steatohepatitis (NASH) phenotype in the liver tissue construct, and wherein the liver tissue construct comprises the NASH phenotype comprising a lipid accumulation, a hepatocellular ballooning, a macrovesicular steatosis, and a histological fibrosis induced by the palmitic acid, oleic acid, or combination thereof.
Dependencies are inferred from the claim text.