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US 12,252,687 B2Granted
Recombinant yeast and use thereof
AssigneesTianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences
InventorsXueli Zhang, Luqi Huang, Zhubo Dai, Dong Wang, Lili Zhang, Juan Guo, Yi Liu
Life of this patent
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Abstract
Provided is a recombinant yeast expressing germacrene A synthetase or a fusion protein thereof, wherein the fusion protein is germacrene A synthetase and farnesyl pyrophosphate synthase. The recombinant yeast improves the yield of germacrene A, and is suitable for the industrialized production of β-elemene and/or germacrene A.
Claims25
A recombinant yeast strain, comprising:
- a fusion protein
- comprising germacrene A synthetase and farnesyl pyrophosphate synthase, wherein the fusion protein is one or more selected from the group consisting of:
- (1) SynSmFPS-GGGS (SEQ ID NO:
- 15)-STpGMAS,
- (2) SynSmFPS-PGGH (SEQ ID NO:
- 16)-STpGMAS,
- (3) SynSmFPS-YRSQI (SEQ ID NO:
- 17)-STpGMAS,
- (4) SynSmFPS-VIPFIS (SEQ ID NO:
- 18)-STpGMAS,
- (5) SynSmFPS-FLYLKF (SEQ ID NO:
- 19)-STpGMAS,
- (6) SynSmFPS-WRFSPKLQ (SEQ ID NO:
- 20)-STpGMAS,
- (7) SynSmFPS-HHVQESQCISTV (SEQ ID NO:
- 21)-STpGMAS, and
- (8) a combination of SynSmFPS-WRFSPKLQ (SEQ ID NO:
- 20)-STpGMAS, ERG20-GGGS (SEQ ID NO:
- 15)-LsLTSC2 and SynSmFPS-GGGS (SEQ ID NO:
- 15)-STpGMAS.
17 more elements
Dependencies are inferred from the claim text.