Fluidic device, fluidic system, and method for developing three-dimensional cellular constructions

A fluidic device for developing a cellular starting material into a three-dimensional cellular structure, comprising a base body, including

• a chamber extending in an x-direction, a y-direction orthogonal to the x-direction and a z-direction orthogonal to the x-direction and the y-direction, in which a matrix is received, into which the cellular starting material to be developed can be is introduced;
• and
• at least two fluid reservoirs, wherein each of the at least two fluid reservoirs comprises:
• a fluid inlet for introducing a fluid medium into the fluid reservoir;
• a fluid outlet for discharging the fluid medium from the fluid reservoir;
• and
• a separating device which is partially permeable to the fluid medium and which separates one of the at least two fluid reservoirs from the chamber and forms a common plane interface between the one of the at least two fluid reservoirs and the chamber, via which the fluid medium diffuses into the matrix;
• wherein the fluidic device is adapted, using fluid media, to form at least one concentration gradient, at least two mutually orthogonal concentration gradients and/or at least two mutually antiparallel concentration gradients (X1, X2, Y1, Y2) in the matrix which are each essentially homogeneous or deliberately inhomogeneous over at least a section of the extension of the matrix in the z-direction, the adaptation being effected by
• (i) two of the separating devices are present, with each extending in the z-direction and the x-direction and being arranged opposite to one another and being spaced from one another by the chamber in the y-direction;
• and/or
• (ii) two more of the separating devices are present, with each extending in the z-direction and the y-direction and being arranged opposite to one another and being spaced from one another by the chamber in the x-direction.