Method of preparing a hydrogel product
AssigneeGalderma Holding SA
InventorsLars Nord, Hotan Mojarradi, Johan Olsson
Life of this patent
Estimating the term…
Abstract
A method of preparing a hydrogel product comprising crosslinked glycosaminoglycan molecules, comprising the steps of: (a) providing a mixed solution of glycosaminoglycan molecules, a di- or multinucleophilic functional crosslinker, and a mononucleophilic functional graft chain; (b) activating carboxyl groups on the glycosaminoglycan molecules with a coupling agent to form activated glycosaminoglycan molecules; and (c) simultaneously crosslinking the activated glycosaminoglycan molecules and grafting the graft chain to the activated glycosaminoglycan molecules by reacting the nucleophiles with the activated carboxyl groups.
Claims29
A method of preparing a hydrogel product comprising crosslinked glycosaminoglycan molecules, the method comprising:
- (a) providing a mixed solution comprising glycosaminoglycan molecules, a di- or multinucleophilic functional crosslinker, and a mononucleophilic functional graft chain;
- (b) activating carboxyl groups on the glycosaminoglycan molecules with a coupling agent to form activated glycosaminoglycan molecules;
- and
- (c) simultaneously crosslinking the activated glycosaminoglycan molecules with the crosslinker and grafting the graft chain to the activated glycosaminoglycan molecules by reacting nucleophilic groups of the crosslinker and the graft chain with the activated carboxyl groups, wherein the coupling agent and free crosslinker are simultaneously present at a concentration ratio of about 125/0.6 to about 125/0.9.
Dependencies are inferred from the claim text.