Method for purifying antibody or antibody-like molecule
AssigneeKaneka Corporation
InventorsFuminori Konoike, Kazunobu Minakuchi
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Abstract
The objective of the present invention is to provide a method capable of efficiently removing an impurity from an aqueous solution or a suspension comprising an antibody or an antibody-like molecule and the impurity. The method for purifying an antibody or an antibody-like molecule according to the present invention is characterized in treating an aqueous solution or a suspension comprising the antibody or the antibody-like molecule and an impurity with a water-insoluble inorganic compound, wherein the water-insoluble inorganic compound comprises one or more elements selected from magnesium, calcium and aluminum.
Claims20
A method for purifying an antibody or a protein, the method comprising the step of:
- adding a pre-formed water-insoluble inorganic compound to an aqueous solution or a suspension comprising the antibody or the protein and an impurity or filling a column with a pre-formed water-insoluble inorganic compound and passing an aqueous solution or a suspension comprising the antibody or the protein through the column to adsorb the impurity onto said pre-formed water-insoluble inorganic compound and obtain a liquid part comprising the antibody or the protein,
- wherein said pre-formed water-insoluble inorganic compound comprises at least one member selected from the group consisting of magnesium carbonate, magnesium oxide, magnesium phosphate, and aluminum oxide, and
- the antibody or the protein is at least one member selected from the group consisting of polyclonal antibody, monoclonal antibody, human antibody, humanized antibody, chimeric antibody, single-chain antibody, heavy chain antibody, multivalent antibody, Fab, F(ab′), F(ab′)2, Fc, Fc fusion protein, bispecific antibody, heavy chain, light chain, single-chain Fv, sc(Fv)2, disulfide bonded Fv, Diabody or micro-antibody.
Dependencies are inferred from the claim text.