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US 12,251,685 B2Granted
Catalytically active substances
AssigneeIllumina, Inc.
InventorLewis J. Kraft
Life of this patent
Estimating the term…
Abstract
A catalytically active substance includes a copper (I) sulfide mineral particle, and an alkyne functionalized molecule bound to a surface of the copper (I) sulfide mineral particle. In an example method, a copper (I) sulfide mineral is reacted with an alkyne functionalized molecule to form a catalytically active substance. The catalytically active substance is reacted with an azide functionalized molecule to couple the catalytically active substance with the azide functionalized molecule.
Claims22
A method of making a triazole, comprising:
- forming a catalytically active substance by reacting, in a mixture, i) a copper (I) sulfide mineral selected from the group consisting of chalcocite, djurleite, and digenite, each in its naturally occurring form not stabilized with a surface polymer or other stabilizing compound and having an average particle size ranging from about 500 nm to about 45 μm with ii) an alkyne functionalized molecule, under conditions allowing direct binding of the alkyne functionalized molecule to a surface of the copper (I) sulfide mineral by a coordinate bond, thereby forming the catalytically active substance;
- and
- reacting the catalytically active substance with an azide functionalized molecule;
- wherein a concentration of the copper (I) sulfide mineral in the mixture is in stoichiometric excess with respect to the alkyne functionalized molecule.
1 more element
A method of grafting a primer to a flow cell surface, comprising:
- forming a catalytically active substance by reacting, in a mixture, i) a copper (I) sulfide mineral selected from the group consisting of chalcocite, djurleite, and digenite, each in its naturally occurring form not stabilized with a surface polymer or other stabilizing compound and having an average particle size ranging from about 500 nm to about 45 μm with ii) an alkyne functionalized primer, under conditions allowing direct binding of the alkyne functionalized primer to a surface of the copper (I) sulfide mineral by a coordinate bond, thereby forming the catalytically active substance;
- and
- reacting the catalytically active substance with an azide functionalized molecule on the flow cell surface, thereby grafting the primer to the flow cell surface;
- wherein a concentration of the copper (I) sulfide mineral in the mixture is in stoichiometric excess with respect to the alkyne functionalized molecule.
1 more element
Dependencies are inferred from the claim text.