Back to results
US 12,251,404 B2Granted
Methods and compositions to maintain stem cell quiescence
AssigneeThe Board of Trustees of the Leland Stanford Junior University
InventorsMarco Quarta, Thomas A. Rando
Life of this patent
Estimating the term…
Abstract
Compositions and methods are provided for induction and maintenance of quiescence of stem cells.
Claims17
A method of culturing cells, comprising:
- culturing ex vivo an isolated population of cells in a medium comprising four or more components selected from the group consisting of elcatonin, forskolin, insulin-transferrin-selenium, MGCD-265, JNJ-7706621, SB203580, SU5402, somatostatin, TGFβ, and a serum replacement for a period of at least 6 hours.
A method of inducing or maintaining quiescence of an isolated population of cells, comprising:
- culturing ex vivo an isolated population of cells in a medium comprising four or more components selected from the group consisting of elcatonin, forskolin, insulin-transferrin-selenium, MGCD-265, JNJ-7706621, SB203580, SU5402, somatostatin, TGFβ, and a serum replacement for a period of at least 6 hours.
A composition, comprising:
- three or more components selected from the group consisting of elcatonin, forskolin, insulin-transferrin-selenium, SB203580, SU5402, TGFβ, and a serum replacement.
A composition, comprising:
- an isolated population of cells in a culture medium, the culture medium comprising three or more components selected from the group consisting of elcatonin, forskolin, insulin-transferrin-selenium, SB203580, SU5402, TGFβ, and a serum replacement.
Dependencies are inferred from the claim text.