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US 12,247,979 B2Granted
Compositions and methods for capturing exosomes
OwnerGale W. Newman, Samuel Anyanwu +1inventors
InventorsGale W. Newman, Samuel Anyanwu, James W. Lillard, Jr.
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Abstract
Compositions and kits for exosome capture include an antibody specifically binding the human asparagine-linked glycosylation 6 homolog (ALG6). The compositions and kits may be employed in a method for diagnosing a disease or condition in a subject.
Claims12
A method for diagnosing a disease or a condition in a subject, comprising:
- (a) contacting a bodily fluid sample from the subject with rabbit anti-human ALG6 polyclonal antibodies under conditions suitable for forming complexes comprising exosomes bound to the polyclonal antibodies,
- (b) contacting the complexes with at least one disease- or condition-associated biomarker binding agent suitable for detecting at least one disease- or condition-associated biomarkers present in the exosomes;
- and
- (c) determining whether the exosomes comprise at least one detectable disease- or condition-associated biomarker,
- wherein detection of the at least one disease- or condition-associated biomarker in step (c) is indicative of a disease or condition in the subject and wherein lack of detection of the at least one disease- or condition-associated biomarker in step (c) is indicative of the absence of the disease or condition in the subject,
- wherein the rabbit anti-human ALG6 polyclonal antibodies are made by administering an Ac-YEAQRHWQEIT (SEQ ID NO:1)-Ahx-C synthetic peptide conjugate to a rabbit and purifying the resultant antibodies.
3 more elements
Dependencies are inferred from the claim text.