Biocatalysts and methods for hydroxylation of chemical compounds
AssigneeCodexis, Inc.
InventorsHaibin Chen, Yong Koy Bong, Fabien L. Cabirol, Anupam Gohel Prafulchandra, Tao Li, Jeffrey C. Moore, Martina Quintanar-Audelo, Yang Hong, Steven J. Collier, Derek Smith
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Abstract
The present disclosure provides engineered proline hydroxylase polypeptides for the production of hydroxylated compounds, polynucleotides encoding the engineered proline hydroxylases, host cells capable of expressing the engineered proline hydroxylases, and methods of using the engineered proline hydroxylases to prepare compounds useful in the production of active pharmaceutical agents.
Claims12
An engineered polypeptide having proline hydroxylase activity, comprising an amino acid sequence having at least 95% sequence identity to SEQ ID NO:2 and having a residue difference at residue position X25.
Dependencies are inferred from the claim text.