Non-covalent loading of plant picovirus particles
AssigneeCase Western Reserve University
InventorsNicole Steinmetz, Daniel Popkin
Life of this patent
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Abstract
A method of non-covalently loading a plant picornavirus is described. The method includes contacting a plant picornavirus in solution with a molar excess of a cargo molecule to load the plant picornavirus with the cargo molecule, and then purifying the loaded plant picornavirus. Examples of cargo molecules include imaging agents, antitumor agents, and antiviral agents. Loaded plant picornaviruses prepared in this manner can be used to delivering cargo molecule to cells.
Claims19
A nanoparticle comprising a native plant picornavirus that has been loaded with one or more cargo molecules, the one or more cargo molecules non-covalently infused into the interior of the native plant picornavirus, wherein the native plant picornavirus is loaded by contacting the native plant picornavirus in solution with a molar excess of at least about 500-fold of the cargo molecule, wherein the cargo molecule is loaded into the native plant picornavirus via simple diffusion from the solution into the picornavirus and purifying the loaded native plant picornavirus.
Dependencies are inferred from the claim text.