Quality assurance of chondrocytes
AssigneeXintela AB
InventorsEvy Lundgren Åkerlund, Katarzyna Chmielarska Masoumi
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Abstract
The present invention relates to methods that are based on expression of integrin alpha10 and integrin alpha11 on chondrocytes, used for determining purity, quality, degree of chondrocytic identity, chondrocytic potency, and/or degree of chondrocytic phenotype of a composition comprising chondrocytes, as well as for isolating and enriching a population of high quality chondrocytes and controlling culturing and expanding of high quality chondrocytes. The present invention relates also to composition comprising chondrocytes.
Claims21
A method for determining quality of a composition comprising isolated and cultured chondrocytes, wherein the quality determination comprises one or more of:
- identity of the chondrocytes, differentiation state of the chondrocytes, purity of the chondrocytes, and potency of the chondrocytes, the method comprising:
- a) providing a candidate composition comprising isolated and cultured chondrocytes;
- b) detecting the expression levels of integrin alpha10 and integrin alpha11 in said composition;
- c) comparing the expression level of integrin alpha10 to the expression level of integrin alpha11;
- and
- d) determining the ratio of integrin alpha10 expression to integrin alpha11 expression;
- wherein detecting an integrin alpha10 to integrin alpha11 expression ratio of at least 4 to 1 is indicative of chondrocytic identity of the chondrocytes in the composition, differentiated state of the chondrocytes in the composition, purity of the chondrocytes in the composition, and/or potency of the chondrocytes of the composition, and thereby is indicative of a high quality chondrocyte composition.
A method for selecting a high quality composition comprising chondrocytes, wherein the quality determination comprises one or more of:
- identity of the chondrocytes, differentiation state of the chondrocytes, purity of the chondrocytes, and potency of the chondrocytes;
- the method comprising:
- a) providing a candidate composition comprising isolated and cultured chondrocytes;
- b) detecting the expression levels of integrin alpha10 and integrin alpha11 in said composition;
- c) comparing the expression levels of integrin alpha10 and integrin alpha11 in said composition;
- and
- d) selecting the chondrocytes having an integrin alpha10 to integrin alpha11 expression ratio of at least 4 to 1.
A method for optimizing chondrocytic potency of an isolated and cultured composition comprising chondrocytes, the method comprising inducing expression of integrin alpha10.
A method for isolating or manufacturing a population of high quality chondrocytes, the method comprising the steps of:
- a) providing a candidate composition comprising chondrocytes;
- b1) enriching chondrocytes having an integrin alpha10 to integrin alpha11 expression ratio of at least 4 to 1, thus obtaining an isolated population of high quality chondrocytes;
- or
- b2) expanding chondrocytes having an integrin alpha10 to integrin alpha11 expression ratio of at least 4 to 1, thus manufacturing a population of high quality chondrocytes.
Dependencies are inferred from the claim text.