3D printed micro-millifluidic bioreactors for long-term retinal organoid maintenance

A bioreactor device comprising:

• a solid substrate having a first face and a second face, the solid substrate at least partially defining a perfusion channel, a plurality of chambers, a fluidic inlet, and a fluidic outlet;
• a first sheet disposed over the first face;
• and
• a second sheet disposed over the second face, wherein the combination of the solid substrate, the first sheet and the second sheet defining the perfusion channel and each chamber of the plurality of chambers, the plurality of chambers being arranged in rows of chambers, the perfusion channel extending from the fluidic inlet and the fluidic outlet with a serpentine path that weaves through each row of chambers such that adjacent chambers are positioned at opposite sides of the perfusion channel, wherein each chamber has an associated connecting channel that provides fluid communication between each chamber and the perfusion channel, each associated connecting channel defining a distance from the perfusion channel that eliminates fluidic movement in each chamber and reduces shear stress;
• and
• a plurality of mixers positioned in the perfusion channel wherein at least one mixer is disposed between adjacent chambers.