Expandable cell populations from brain biopsies of living subjects
AssigneeLondon Health Sciences Centre Research Inc.
InventorMatthew Olding Hebb
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Abstract
The present invention relates to a method of producing expandable cultured brain cells. The brain cells are neurotrophic factor (NTF) positive. The expandable cultured brain cells are obtained by culturing a biopsy obtained from the cortical and/or subcortical brain region of a living subject. The biopsies can be obtained during neurosurgical procedures such as deep brain stimulation. The expandable cultured brain cells of the present invention are useful for the treatment of neurological diseases and other medical conditions.
Claims15
A method of producing a population of expanded glial progenitor brain cells characterized as expressing nestin, an oligodendrogial protein and a mesenchymal protein and that express at least one neurotrophic factor (NTF) selected from a glial cell line-derived neurotrophic factor (GDNF), a brain-derived neurotrophic factor (BDNF), or a cerebral dopamine neurotrophic factor (CDNF), the method comprising:
- (a) culturing brain cells of a cortical and/or subcortical brain regions of a living subject under conditions suitable for cell expansion to obtain cultured cells,
- (b) testing the cultured cells for expression of nestin, the oligodendroglial protein, the mesenchymal protein and the at least one NTF, and
- (c) isolating the cultured cells that express the nestin, oligodendroglial protein, the mesenchymal protein and the at least one NTF thereby producing the population of expanded progenitor brain cells that express nestin, oligodendroglial protein, the mesenchymal protein and the at least one NTF.
Dependencies are inferred from the claim text.