Devices and methods for sensing and sorting particles

A microfluidic device, comprising:

• a substrate with a microfluidic channel having an inlet, a first region, an expansion region, and a second region, the microfluidic channel being coupled with two or more output channels, wherein:
• the first region has a first width;
• the second region is downstream from the first region and upstream from the two or more output channels;
• and
• the expansion region is between the first region and the second region, has a second width that is greater than the first width, and is configured to reduce a velocity of respective particles flowing through the microfluidic channel;
• one or more sensors located adjacent to the first region of the microfluidic channel for sensing the respective particles;
• an inlet piezoelectric actuator located adjacent to the inlet and configured to facilitate input mixing of samples;
• a first piezoelectric actuator located adjacent to the second region of the microfluidic channel and configured to deflect the respective particles to respective output channels of the two or more output channels based on signals from the one or more sensors;
• and
• a set of one or more outlet piezoelectric actuators located adjacent to at least one of the two or more output channels and configured to facilitate ejection of the respective particles.