A method of encapsulating active ingredients in liposomes, comprising:
- a solution of the active ingredient;
- and
- at least one bilayer composed of two at least monomolecular layers of at least one first amphiphilic compound;
- wherein the active ingredient solution is encapsulated by the at least one bilayer, comprising the following steps:
- (a) providing the active ingredient solution of the active ingredient to be encapsulated by dissolving the active ingredient in at least one solvent;
- (b) providing a first emulsion by emulsifying the active ingredient solution from step (a) in at least one first liquid having zero or sparing miscibility with the at least one solvent of the active ingredient solution, in the presence of the at least one first amphiphilic compound, in order to accumulate an at least monomolecular inner layer of the at least one first amphiphilic compound onto droplets of the active ingredient solution emulsified in the at least one first liquid;
- (c) providing a liquid phase having zero or sparing miscibility with the at least one first liquid of the first emulsion from step (b);
- (d) contacting the first emulsion composed of the at least one first liquid and having the droplets of the active ingredient solution that are emulsified therein and have the at least monomolecular inner layer of the at least one first amphiphilic compound that has been accumulated thereon from step (b) with the liquid phase from step (c) to form a phase boundary between the first emulsion from step (b) and the liquid phase from step (c), wherein the at least one first amphiphilic compound is enriched at the phase boundary;
- and
- (e) centrifuging the first emulsion from step (b) and the liquid phase from step (c) that are in contact with one another via the phase boundary, in order to transfer the droplets of the active ingredient solution present in the first emulsion and having the at least monomolecular inner layer of the at least one first amphiphilic compound that has been accumulated thereon from the at least one first liquid of the first emulsion from step (b) into the liquid phase from step (c), wherein, when the droplets of the active ingredient solution having the at least monomolecular inner layer of the at least one first amphiphilic compound that has been accumulated thereon passes the phase boundary, the at least one first amphiphilic compound enriched there is accumulated on the at least monomolecular inner layer of the at least one first amphiphilic compound of the droplets of the active ingredient solution to form an at least monomolecular outer layer thereof, in order to produce the at least one bilayer composed of the two at least monomolecular layers of the at least one first amphiphilic compound;
- wherein the at least one first liquid of the first emulsion from step (b) is chosen such that the solubility of the at least one first amphiphilic compound in the at least one first liquid is not more than 1×10−4 mol/l.