D-glucaric acid producing bacterium, and method for manufacturing D-glucaric acid

A method for producing D-glucaric acid and a salt thereof, which comprises

• obtaining an alcohol dehydrogenase ADH (1) and an aldehyde dehydrogenase ALDH (2) in the form of
• (a) a cultivated strain of Pseudogluconobacter saccharoketogenes Rh47-3 that expresses the alcohol dehydrogenase ADH (1) and the aldehyde dehydrogenase ALDH (2), said cultivated strain has reduced or eliminated activity of an aldehyde dehydrogenase ALDH (3), or
• (b) a processed product of the cultivated strain,
• mixing the cultivated strain or the processed product with an aqueous solution of one or more saccharides selected from the group consisting of D-glucose, D-gluconic acid, and L-guluronic acid,
• adjusting the saccharide concentration within the range of 1 to 5% (w/v),
• adding one or more alkali compounds selected from the group consisting of sodium hydroxide, potassium hydroxide, and calcium carbonate, whereby producing D-glucaric acid from L-guluronic acid without generating D-glucaraldehyde or D-glucuronic acid, and
• recovering the D-glucaric acid or the salt thereof resulting therefrom.