Cytochrome P450 monooxygenase catalyzed oxidation of sesquiterpenes
OwnerMichel Schalk, Letizia Rocci +1inventors
InventorsMichel Schalk, Letizia Rocci, Fabienne Deguerry
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Abstract
Described herein are the nucleic acid and the amino acid sequences of a cytochrome P450 capable of oxidizing terpene molecules. Also described herein are methods of oxidizing terpene molecules including contacting the cytochrome P450 with the terpene molecule intended to be oxidized. In particular, the method may be carried out in vitro or in vivo to produce oxidized terpene molecules, which may be used in different technical fields such as for example perfumery and flavoring. Also described herein is an expression vector containing the nucleic acid. A non-human host organism or a cell transformed with the nucleic acid is also an object of the disclosure.
Claims9
A method for producing an oxidized zizaene compound, which method comprises
- a. contacting zizaene or a zizaene containing composition with a polypeptide having
- Cytochrome P450 monooxygenase activity, selected from:
- i. VzCP8201 comprising an amino acid sequence having at least 90% sequence identity to SEQ ID NO:
- 19,
- ii. VzCP521-11 comprising an amino acid sequence having at least 90% sequence identity to SEQ ID NO:
- 21,
- iii. VzCP7186 comprising an amino acid sequence having at least 90% sequence identity to SEQ ID NO:
- 20,
- iv. or combinations of at least two of said polypeptides;
- thereby predominantly obtaining at least one oxidation product of zizaene, wherein the at least one oxidation product of zizaene comprises zizaenol, wherein the polypeptide catalyzes an oxidation reaction of zizaene in position C3;
- b. optionally isolating a zizaene oxidation product as obtained in step a;
- and
- c. converting the zizaene oxidation product to zizaenone, or to a mixture comprising zizaenone and epi-zizaenone.
Dependencies are inferred from the claim text.