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US 12,239,704 B2Granted
Recombinant non-pathogenic Marek's disease virus constructs encoding multiple heterologous antigens
AssigneeIntervet Inc.
InventorsStephanie M. Cook, Mohamad Morsey, Ian Tarpey
Life of this patent
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Abstract
The present invention discloses novel recombinant multivalent non-pathogenic Marek's Disease virus constructs that encode and express foreign antigens from three or more avian viruses, along with methods of the use of the multivalent poultry virus vaccines.
Claims12
A recombinant herpesvirus of turkeys (rHVT) that comprises a first heterologous nucleic acid and a second heterologous nucleic acid;
- wherein the first heterologous nucleic acid is located in a first nonessential site in the rHVT genome, and the second heterologous nucleic acid is located in a second nonessential site in the rHVT genome;
- wherein the first nonessential site is the UL54.5 site and the second nonessential site is the US2 site;
- wherein the first heterologous nucleic acid encodes an Infectious Bursal Disease Virus viral protein 2 (IBDV VP2), an Infectious Laryngotracheitis Virus glycoprotein D (ILTV gD) and an Infectious Laryngotracheitis Virus glycoprotein I (ILTV gI), and wherein the second heterologous encodes a Newcastle Disease Virus fusion protein (NDV F);
- and
- wherein the first heterologous nucleic acid is configured to be transcribed in the opposite direction relative to a UL54.5 gene of the rHVT.
2 more elements
A recombinant herpesvirus of turkeys (rHVT) that comprises a first heterologous nucleic acid that comprises the nucleotide sequence of SEQ ID NO:
- 21 and a second heterologous nucleic acid that comprises the nucleotide sequence of SEQ ID NO:
- 22, wherein the first heterologous nucleic acid comprises an Infectious Bursal Disease Virus viral protein 2 (IBDV VP2) gene, an Infectious Laryngotracheitis Virus glycoprotein D (ILTV gD) gene and an Infectious Laryngotracheitis Virus glycoprotein I (ILTV gI) gene configured to be transcribed in the opposite direction relative to a UL54.5 gene of the rHVT.
Dependencies are inferred from the claim text.