Plant body of genus Nicotiana with low alkaloid content and production method thereof
AssigneeJapan Tobacco Inc.
InventorsTakanori Takeuchi, Masao Arai, Hisashi Udagawa, Hiroshi Magome, Ryo Nishiguchi, Yoshimitsu Takakura
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Abstract
An embodiment of the present invention provides a tobacco plant having a low alkaloid content. In the tobacco plant, a function of a gene encoding aspartate oxidase AO2 is suppressed.
Claims17
A tobacco plant in which a mutation that specifically causes suppression of a function of an endogenous gene is introduced in the endogenous gene in a genome, the endogenous gene being at least one of:
- (a) an endogenous gene which contains, as a coding region, a polynucleotide that encodes a polypeptide having a sequence identity of 95% or higher with an amino acid sequence represented by SEQ ID NO:
- 2 or SEQ ID NO:
- 5;
- and
- (b) an endogenous gene which contains, as a coding region, a polynucleotide that encodes a polypeptide having a sequence identity of 95% or higher with an amino acid sequence represented by SEQ ID NO:
- 6;
- wherein respective functions of the following endogenous genes (c) and (d) are not suppressed:
- (c) an endogenous gene which contains, as a coding region, a polynucleotide that encodes a polypeptide having a sequence identity of 95% or higher with an amino acid sequence represented by SEQ ID NO. 62 or SEQ ID NO:
- 63;
- and
- (d) an endogenous gene which contains, as a coding region, a polynucleotide that encodes a polypeptide having a sequence identity of 95% or higher with an amino acid sequence represented by SEQ ID NO. 64.
A method of producing a tobacco plant, comprising the step of introducing a mutation in a genome of a tobacco plant, the mutation specifically causing suppression of a function of at least one of:
- (a) an endogenous gene which contains, as a coding region, a polynucleotide that encodes a polypeptide having a sequence identity of 95% or higher with an amino acid sequence represented by SEQ ID NO:
- 2 or SEQ ID NO:
- 5;
- and
- (b) an endogenous gene which contains, as a coding region, a polynucleotide that encodes a polypeptide having a sequence identity of 95% or higher with an amino acid sequence represented by SEQ ID NO:
- 6;
- wherein,
- the step of introducing the mutation including introducing the mutation in the at least one of the endogenous genes, and wherein respective functions of the following endogenous genes (c) and (d) are not suppressed:
- (c) an endogenous gene which contains, as a coding region, a polynucleotide that encodes a polypeptide having a sequence identity of 95% or higher with an amino acid sequence represented by SEQ ID NO. 62 or SEQ ID NO:
- 63;
- and
- (d) an endogenous gene which contains, as a coding region, a polynucleotide that encodes a polypeptide having a sequence identity of 95% or higher with an amino acid sequence represented by SEQ ID NO. 64.
A cured leaf having a mutation in (a) an endogenous gene in a genome which endogenous gene contains, as a coding region, a polynucleotide that encodes a polypeptide having a sequence identity of 95% or higher with an amino acid sequence represented by SEQ ID NO:
- 2 or SEQ ID NO:
- 5, the mutation suppressing a function of the endogenous gene, and/or
- having a mutation in (b) an endogenous gene in a genome which endogenous gene contains, as a coding region, a polynucleotide that encodes a polypeptide having a sequence identity of 95% or higher with an amino acid sequence represented by SEQ ID NO:
- 6, the mutation suppressing a function of the endogenous gene, and
- having a lower alkaloid content as compared with a cured leaf produced from a tobacco leaf harvested from a wild-type tobacco plant;
- wherein respective functions of the following endogenous genes (c) and (d) are not suppressed:
- (c) an endogenous gene which contains, as a coding region, a polynucleotide that encodes a polypeptide having a sequence identity of 95% or higher with an amino acid sequence represented by SEQ ID NO. 62 or SEQ ID NO:
- 63;
- and
- (d) an endogenous gene which contains, as a coding region, a polynucleotide that encodes a polypeptide having a sequence identity of 95% or higher with an amino acid sequence represented by SEQ ID NO. 64.
Dependencies are inferred from the claim text.