Chromatography method, method of determining the concentration of at least one compound in a chromatography method, method of obtaining an adsorption isotherm, method of obtaining at least one stationary phase and method of evaluating the accuracy of a predetermined adsorption isotherm

A method of obtaining an adsorption isotherm of at least one compound comprising the steps of:

• (ia) selecting at least one compound;
• (ib) selecting a stationary phase;
• (ic) selecting a mobile phase;
• (id) selecting a temperature of the at least one compound, the stationary phase and the mobile phase;
• wherein at least one parameter, selected from parameters capable of characterizing the composition of the mobile phase and the temperature, varies;
• (ii) obtaining a plurality of parameter value sets including the at least one varying parameter and the concentration of the at least one compound in the mobile phase;
• (iii) obtaining a plurality of binding capacity values of the at least one compound by the stationary phase for each of the plurality of parameter value sets obtained in step (ii);
• and
• (iv) obtaining the adsorption isotherm based on the plurality of binding capacity values obtained in step (iii) by means of multivariate data analysis,
• wherein the adsorption isotherm obtained in step (iv) is an equation having the form q=f(c,pj) that relates the binding capacity q of a compound by the stationary phase to the concentration c of the at least one compound in the mobile phase and to at least one further parameter pj selected from parameters characterizing the composition of the mobile phase and the temperature.