Back to results
US 12,234,511 B2Granted
Methods of analyte detection
AssigneePacific Biosciences of California, Inc.
InventorsBryan P. Staker, Niandong Liu, Michael David McLaughlin, Bart Lee Staker
Life of this patent
Estimating the term…
Abstract
Disclosed herein are methods and systems for detection and discrimination of optical signals from a densely packed substrate. These have broad applications for biomolecule detection near or below the diffraction limit of optical systems, including in improving the efficiency and accuracy of polynucleotide sequencing applications.
Claims20
A method of identifying an analyte, comprising:
- (a) providing at least one analyte of a plurality of analytes, wherein said at least one analyte of said plurality of analytes is immobilized on a substrate;
- (b) performing a plurality of cycles of binding one or more probes to said at least one analyte of said plurality of analytes, wherein said plurality of cycles of probe binding generates a sequence of optical signals;
- (c) detecting said sequence of optical signals with an imaging system, wherein said imaging system has about 2 pixels per μm2 of said substrate;
- and
- (d) processing said sequence of optical signals with an expected sequence of optical signals corresponding to said at least one analyte of said plurality of analytes to generate a decoded sequence of optical signals, wherein said decoded sequence of optical signals identifies said at least one analyte of said plurality of analytes with an error rate less than 5% over at least 20 cycles of said plurality of cycles.
2 more elements
A system for identifying an analyte, comprising:
- one or more processors and a memory storing one or more programs for execution by said one or more processors, said one or more programs comprising instructions to:
- (i) perform a plurality of cycles of binding one or more probes to at least one analyte of a plurality of analytes, wherein said at least one analyte of said plurality of analytes is immobilized on a substrate, and wherein said plurality of cycles of probe binding generates a sequence of optical signals;
- (ii) detect said sequence of optical signals with an imaging system, wherein said imaging system has about 2 pixels per um2 of said substrate;
- and
- (iii) process said sequence of optical signals with an expected sequence of optical signals corresponding to said at least one analyte of said plurality of analytes to generate a decoded sequence of optical signals, wherein said decoded sequence of optical signals identifies said at least one analyte of said plurality of analytes with an error rate less than 5% over at least 20 cycles of said plurality of cycles.
2 more elements
Dependencies are inferred from the claim text.