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US 12,234,493 B2Granted
Compositions for saccharification of cellulosic material
AssigneeNovozymes, Inc.
InventorsBrett McBrayer, Elena Vlasenko, Tarana Shaghasi
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Abstract
The present invention relates to enzyme compositions for high temperature saccharification of cellulosic material and to uses thereof.
Claims55
An isolated recombinant host cell encoding an enzyme composition comprising:
- (I) a polypeptide having cellobiohydrolase I activity selected from the group consisting of:
- (a) a polypeptide comprising an amino acid sequence having at least 85% sequence identity to the mature polypeptide of SEQ ID NO:
- 158;
- (b) a polypeptide encoded by a polynucleotide that hybridizes under at least high stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO:
- 157, (ii) the genomic DNA sequence of the mature polypeptide coding sequence of SEQ ID NO:
- 157, or (iii) the full-length complement of (i) or (ii), wherein high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 micrograms/ml sheared and denatured salmon sperm DNA, and 50% formamide and washing three times each for 15 minutes using 2×SSC, 0.2% SDS at 65° C.;
- (c) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence having at least 85% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
- 5;
- and (d) a polypeptide comprising the mature polypeptide of SEQ ID NO:
- 6;
- (II) a polypeptide having beta-glucosidase activity;
- and
- (III) a polypeptide having endoglucanase I activity is selected from the group consisting of:
- (a) a polypeptide comprising an amino acid sequence having at least 85% sequence identity to the mature polypeptide of SEQ ID NO:
- 62;
- (b) a polypeptide encoded by a polynucleotide that hybridizes under at least high stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO:
- 61, (ii) the genomic DNA sequence of the mature polypeptide coding sequence of SEQ ID NO:
- 61, or (iii) the full-length complement of (i) or (ii), wherein high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 micrograms/ml sheared and denatured salmon sperm DNA, and 50% formamide and washing three times each for 15 minutes using 2×SSC, 0.2% SDS at 65° C.;
- (c) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence having at least 85% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
- 61;
- and (d) a polypeptide comprising the mature polypeptide of SEQ ID NO:
- 62.
19 more elements
A method for degrading or converting a cellulosic material, comprising:
- treating the cellulosic material with an enzyme composition comprising:
- (I) a polypeptide having cellobiohydrolase I activity selected from the group consisting of:
- (a) a polypeptide comprising an amino acid sequence having at least 85% sequence identity to the mature polypeptide of SEQ ID NO:
- 158;
- (b) a polypeptide encoded by a polynucleotide that hybridizes under at least high stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO:
- 157, (ii) the genomic DNA sequence of the mature polypeptide coding sequence of SEQ ID NO:
- 157, or (iii) the full-length complement of (i) or (ii), wherein high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 micrograms/ml sheared and denatured salmon sperm DNA, and 50% formamide and washing three times each for 15 minutes using 2×SSC, 0.2% SDS at 65° C.;
- (c) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence having at least 85% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
- 5;
- and (d) a polypeptide comprising the mature polypeptide of SEQ ID NO:
- 6;
- (II) a polypeptide having beta-glucosidase activity;
- and
- (III) a polypeptide having endoglucanase I activity is selected from the group consisting of:
- (a) a polypeptide comprising an amino acid sequence having at least 85% sequence identity to the mature polypeptide of SEQ ID NO:
- 62;
- (b) a polypeptide encoded by a polynucleotide that hybridizes under at least high stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO:
- 61, (ii) the genomic DNA sequence of the mature polypeptide coding sequence of SEQ ID NO:
- 61, or (iii) the full-length complement of (i) or (ii), wherein high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 micrograms/ml sheared and denatured salmon sperm DNA, and 50% formamide and washing three times each for 15 minutes using 2×SSC, 0.2% SDS at 65° C.;
- (c) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence having at least 85% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
- 61;
- and (d) a polypeptide comprising the mature polypeptide of SEQ ID NO:
- 62;
- wherein the degrading or converting of the cellulosic material is performed with the enzyme composition at a temperature in the range of about 40° C. to about 70° C.
21 more elements
A method for producing a fermentation product, comprising:
- (a) saccharifying a cellulosic material with an enzyme composition comprising:
- (I) a polypeptide having cellobiohydrolase I activity selected from the group consisting of:
- (a) a polypeptide comprising an amino acid sequence having at least 85% sequence identity to the mature polypeptide of SEQ ID NO:
- 158;
- (b) a polypeptide encoded by a polynucleotide that hybridizes under at least high stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO:
- 157, (ii) the genomic DNA sequence of the mature polypeptide coding sequence of SEQ ID NO:
- 157, or (iii) the full-length complement of (i) or (ii), wherein high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 micrograms/ml sheared and denatured salmon sperm DNA, and 50% formamide and washing three times each for 15 minutes using 2×SSC, 0.2% SDS at 65° C.;
- (c) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence having at least 85% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
- 5;
- and (d) a polypeptide comprising the mature polypeptide of SEQ ID NO:
- 6;
- (II) a polypeptide having beta-glucosidase activity;
- and
- (III) a polypeptide having endoglucanase I activity is selected from the group consisting of:
- (a) a polypeptide comprising an amino acid sequence having at least 85% sequence identity to the mature polypeptide of SEQ ID NO:
- 62;
- (b) a polypeptide encoded by a polynucleotide that hybridizes under at least high stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO:
- 61, (ii) the genomic DNA sequence of the mature polypeptide coding sequence of SEQ ID NO:
- 61, or (iii) the full-length complement of (i) or (ii), wherein high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 micrograms/ml sheared and denatured salmon sperm DNA, and 50% formamide and washing three times each for 15 minutes using 2×SSC, 0.2% SDS at 65° C.;
- (c) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence having at least 85% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
- 61;
- and (d) a polypeptide comprising the mature polypeptide of SEQ ID NO:
- 62;
- wherein the saccharification of the cellulosic material is performed with the enzyme composition at a temperature in the range of about 40° C. to about 70° C.;
- (b) fermenting the saccharified cellulosic material with one or more fermenting microorganisms to produce the fermentation product;
- and
- (c) recovering the fermentation product from the fermentation.
24 more elements
Dependencies are inferred from the claim text.