Culture medium for pluripotent stem cells
AssigneePromocell GmbH
InventorHagen Wieland
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Abstract
The present invention relates to a chemically defined medium for eukaryotic cell culture, comprising water, at least one carbon source, one or more vitamins, one or more salts, one or more fatty acids, one or more buffer components, selenium, at least one substance of the group of Functional Inhibitors of Acid Sphingomyelinase (FIASMAs) and at least one polypeptide of the TGF-β superfamily with the ability to inhibit stem cell differentiation, its use in the culture of human pluripotent stem cells, a cell culture system comprising human pluripotent stem cells and the chemically defined medium, as well as a kit for proliferation and/or maintenance of human pluripotent stem cells.
Claims18
A chemically defined medium for eukaryotic cell culture, comprising water, at least one carbon source, one or more vitamins, one or more salts, one or more fatty acids, one or more buffer components, selenium, at least one substance of the group of Functional Inhibitors of Acid Sphingomyelinase (FIASMAs) and at least one polypeptide of the TGF-β superfamily with the ability to inhibit stem cell differentiation,
- wherein the chemically defined medium is free of FGF-2,
- wherein the chemically defined medium is completely devoid of human- or animal-derived substances,
- wherein the ratio between said FIASMA and said at least one polypeptide of the TGF-β superfamily is from 400:1 to 2000:1,
- wherein the concentration of the at least one FIASMA is in the range of 0.1 mg/L to 10 mg/L, and
- wherein the concentration of the at least one polypeptide of the TGF-β superfamily is in the range of 0.2 μg/L to 20 μg/L.
Dependencies are inferred from the claim text.