Method of autologous primary hair follicles preparation in 3D culture

A method for creating de novo autologous primary hair follicles in 3D culture by recreating the conditions of embryogenesis of primary hair follicles, which involves:

• isolating mesenchymal stem cells of donor hair follicles and their expansion;
• isolating of donor scalp skin cells and their temporary cryopreservation;
• seeding, using a sandwich method, mesenchymal stem cells of the donor's hair follicles and skin cells of the donor's scalp in a monolayer of fibrin gel based on a nutrient medium containing growth factors of fetal scalp hair follicles;
• lysing of the fibrin gel monolayer with exogenous plasmin;
• isolating de novo-formed primary donor hair follicles from a lysed fibrin gel monolayer by self-precipitation;
• washing the de novo-formed primary hair follicles of the donor with phosphate buffer;
• transplanting of de novo-formed primary hair follicles to a donor.