Scalable primate pluripotent stem cell aggregate suspension culture
AssigneeViaCyte, Inc.
InventorThomas C Schulz
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Abstract
The present invention relates to methods for production of undifferentiated or differentiated embryonic stem cell aggregate suspension cultures from undifferentiated or differentiated embryonic stem cell single cell suspensions and methods of differentiation thereof.
Claims18
A bioreactor comprising primate pluripotent stem cell (pPSC) aggregates and pPSC-derived cell aggregates in suspension in a differentiation medium, wherein the pPSC-derived cell aggregates are produced in the bioreactor by agitating the pPSC aggregates at a shear rate of 5 to 500 sec−1, wherein the differentiation medium is substantially free of insulin, insulin-like growth factor-1 (IGF-1), or both insulin and IGF-1.
Dependencies are inferred from the claim text.