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US 12,234,476 B2Granted
VCN enhancer compositions and methods of using the same
Assigneebluebird bio, Inc.
InventorsMelissa Bonner, Olivier Negre
Life of this patent
Estimating the term…
Abstract
The invention provides improved gene therapy methods and compositions.
Claims17
A method of increasing the vector copy number (VCN) in a population of CD34+ hematopoietic stem or progenitor cells comprising culturing the cells for at least two hours in the presence of:
- (1) a lentiviral vector at a multiplicity of infection (MOI) of about 10 to about 30;
- (2) a prostaglandin E2(PGE2), or 16,16-dimethyl PGE2 ;
- and (3) poloxamer 338, wherein the increase of VCN achieved by culturing the cells for at least two hours in the presence of the lentiviral vector, poloxamer 338 and one of PGE2 or 16,16-dimethyl PGE2 is further enhanced beyond the increased VCN achieved by culturing the cells for at least two hours in the presence of the lentiviral vector and one of PGE2 or 16,16-dimethyl PGE2 and the absence of poloxamer 338.
Dependencies are inferred from the claim text.