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US 12,234,464 B2Granted
Biosynthesis of mogrosides
AssigneeGinkgo Bioworks, Inc.
InventorsJeffrey Ian Boucher, Nicholas Flores, Jaide Jensen, Dayal Saran, Jue Wang
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Abstract
Provided herein are enzymes (e.g., cucurbitadienol synthases (CDS), UDP-glycosyltransferases (UGT), C11 hydroxylases, epoxide hydrolases (EPH), squalene epoxidases, and/or cytochrome P450 reductases), host cells expressing the enzymes, and methods of producing mogrol precursors, mogrol, and/or mogrosides using such host cells.
Claims19
A host cell that comprises a heterologous polynucleotide encoding a cucurbitadienol synthase (CDS) enzyme, wherein the heterologous polynucleotide sequence is at least 90% identical to SEQ ID NO:
- 3 and/or the amino acid sequence of the CDS encoded by the heterologous polynucleotide is at least 90% identical to SEQ ID NO:
- 43, and wherein the host cell produces 11-hydroxycucurbitadienol, 24-25 epoxy-cucurbitadienol or cucurbitadienol, and wherein the host cell is not a plant cell.
Dependencies are inferred from the claim text.