Back to results
US 12,234,445 B2Granted
Glycogen-null methanotrophs and uses thereof
AssigneeCalysta, Inc.
InventorsRenee M. Saville, Joshua A. Silverman, Vincent Tang, Eric G. Luning, Paloma Rueda, Megan Hsi, Yelena Stegantseva
Life of this patent
Estimating the term…
Abstract
The present disclosure provides methanotrophic bacteria that are modified to produce less glycogen, and methods of using the modified methanotrophic bacteria to produce a desired product, such as protein(s) or metabolite(s).
Claims33
A modified Methylococcus capsulatus, comprising:
- (i) a chromosomal knock-out of a glgA2 isoform of a glycogen synthase gene, or
- (ii) a chromosomal knock-out of an ADP-glucose pyrophosphorylase gene and a chromosomal knock-out of a glgA2 isoform of a glycogen synthase gene, wherein the modified Methylococcus capsulatus cultured under conditions comprising a non-limiting amount of a C1 substrate produces:
- at least about 30% less glycogen as compared to the parent Methylococcus capsulatus cultured under the same conditions;
- and/or
- at least about 5% more crude protein as compared to the parent Methylococcus capsulatus cultured under the same conditions.
2 more elements
Dependencies are inferred from the claim text.