Programmable immunocyte receptor complex system
OwnerJoseph D. Kittle, Joel S. Lwande +2inventors
InventorsJoseph D. Kittle, Joel S. Lwande, Yuanyuan Tang, Shengwen Liang
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Abstract
A programmable receptor complex expressed by an immunocyte, wherein the programmable receptor complex includes a plurality of native or endogenously expressed receptor subunits, wherein the native or endogenously expressed receptor subunits have been engineered or modified to include FcγRI receptor components, biotin-binding components, or both, and wherein the FcγRI receptor components and biotin-binding components are operative to bind to target detector molecules that bind to or otherwise interact with predetermined targets.
Claims18
A target detection system, comprising:
- (a) a modified receptor complex expressed by an immunocyte, wherein the modified receptor complex includes a plurality of receptor subunits including at least one CD3-delta, one CD3-gamma, one TCR alpha one TCR beta, two CD3-zeta and two CD3-epsilon, and wherein at least one of the plurality of receptor subunits has been engineered or modified to include either:
- (i) a biotin-binding component that binds to a target detector molecule that is separate from the receptor complex and that binds to or otherwise interacts with a predetermined target;
- or
- (ii) an FcγRI receptor component that binds to a target detector molecule that is separate from the receptor complex and that binds to or otherwise interacts with a predetermined target;
- and
- (b) a target detector molecule that is not expressed by the immunocyte and that is separate from the receptor complex, wherein the target detector molecule:
- (i) is not an antibody and includes a biotin moiety and a paratope or ligand that is specific for the predetermined target;
- or
- (ii) is an antibody that binds to either to the biotin-binding component or to the FcγRI receptor component and that is specific for the predetermined target.
A target detection system, comprising:
- (a) a modified receptor complex expressed by an immunocyte, wherein the modified receptor complex includes a plurality of T cell receptor subunits including at least one CD3-delta, one CD3-gamma, one TCR alpha one TCR beta, two CD3-zeta and two CD3-epsilon, and wherein at least one of the plurality of T cell receptor subunits has been engineered or modified to include either:
- (i) a biotin-binding component that binds to a target detector molecule that is separate from the receptor complex and that binds to or otherwise interacts with a predetermined target;
- or
- (ii) an FcγRI receptor component that binds to a target detector molecule that is separate from the receptor complex and that binds to or otherwise interacts with a predetermined target;
- and
- (b) a target detector molecule that is not expressed by the immunocyte and that is separate from the receptor complex, wherein the target detector molecule:
- (i) is not an antibody and includes a biotin moiety and a paratope or ligand that is specific for the predetermined target;
- or
- (ii) is an antibody that binds to either to the biotin-binding component or to the FcγRI receptor component and that is specific for the predetermined target.
Dependencies are inferred from the claim text.