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US 12,234,260 B2Granted
Virus-like nanoparticles for oral delivery
AssigneeThe Regents of the University of California
InventorsR. Holland Cheng, Chun Chieh Chen, Mohammad Ali Baikoghli, Marie Stark
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Abstract
A Hepatitis E virus (HEV)-based virus like nanoparticle (HEVNP) made with a modified capsid protein containing at least a portion of open reading frame 2 (ORF2) protein conjugated with gold nanocluster is provided. Also provided are methods of targeted delivery of a nucleic acid using the HEVNP.
Claims19
A composition comprising:
- (i) a modified capsid protein comprising a portion of hepatitis E virus (HEV) open Reading Frame 2 (ORF2) protein, wherein at least one amino acid in the 342-344, 402-408, 510-514, 493-498, 570-579, 529-536, or 520-525 segment of the HEV ORF2 protein amino acid sequence set forth in SEQ ID NO:
- 1 or the corresponding segment of SEQ ID NO:2, 3, 4, 5, or 6 is replaced with a cysteine, which is conjugated with a nanocluster of an element selected from Groups 3 through 18 having an atomic number greater than 20, wherein the nanocluster is about 2-3 nm horizontally and about 2 nm vertically from the cysteine;
- and
- (ii) a bioactive agent encapsulated in an HEV virus-like particle (VLP) formed by the modified capsid protein.
1 more element
Dependencies are inferred from the claim text.