Loading nucleic acids onto substrates
AssigneePacific Biosciences of California, Inc.
InventorsLei Sun, Jaime Juan Benitez-Marzan, Natasha Popovich, Sassan Sheikholeslami, Steven Lin, Aparna Vedula, Michael W. Hunkapiller, Erik Miller, Satwik Kamtekar
Life of this patent
Estimating the term…
Abstract
Methods, compositions, and systems for distributing nucleic acids into array regions are provided. The methods, compositions, and systems utilize nucleic acid condensing agents to increase efficiency of distribution of the nucleic acids into the array regions. Various methods for facilitating distribution of the nucleic acids to the array regions are provided.
Claims27
A method for distributing polymerase-template complexes into a plurality of nanoscale wells, the method comprising:
- contacting polymerase-template complexes in solution with polyethylene glycol PEG) and a salt comprising a cation, thereby providing a first solution;
- providing a surface comprising the plurality of nanoscale wells, wherein the plurality of nanoscale wells are bathed in a second solution comprising a buffer;
- and
- exposing the surface to the first solution, wherein the first solution has a higher density than the second solution, such that the first solution sinks to the bottom of the nanoscale wells and forms a layer underlying the second solution, thereby distributing the polymerase-template complexes into the nanoscale wells.
Dependencies are inferred from the claim text.